D-Luciferin is a common bioluminescent substrate used for imaging and quantification of luciferase expression. It emits a characteristic yellow-green light upon oxidation by luciferase in the presence of cofactors such as ATP, Mg2+ and oxygen.

 

It is a common reagent used in the biotechnology field and specifically for in vivo imaging. Luciferase labeled tumor cells, stem cells or bacteria are typically injected into research animals such as rats or mice. The injection of luciferin allows for the real-time, noninvasive, and longitudinal monitoring of disease progression and/or drug efficacy in these model systems using ultrasensitive bioluminescent imaging.

 

Luciferin is also commonly used for in vitro research, including luciferase and ATP assays, gene reporter assays, high throughput sequencing and various bacteria contamination assays. In addition to D-luciferin, we offer a set of unique caged luciferin bioluminescent substrates for imaging of a wide variety of biological processes (see bioluminescent substrates for more information).